Dual Centrifugation – a new way for fast, safe, and controlled production of liposomes

On Wednesday, June 28, 2023, an online seminar on "Dual Centrifugation (DC) – a new way for fast, safe, and controlled production of liposomes" was held at 3.30 p.m. (CEST).

Here you can  download the presentation.

The speaker was Professor Dr. Ulrich Massing from the University of Freiburg and Andreas Hettich GmbH & Co. KG, Germany.


Dual centrifugation (DC) is a rather new in-vial homogenization technique process in which a sample vial in a fast-running centrifuge is additionally turned around a second axis. Due to that, the direction of the high centrifugal acceleration continuously changes in relation to the (turning) sample vial, which results in high frequent and very strong movements of the sample material inside the vial, resulting in efficient homogenization.

DC was first used for the preparation of liposomes in 2008. Since then, DC has continuously been developed for preparing liposomes and other lipid nanoparticles including emulsions, solid lipid nanoparticles or polymersomes. Significant improvements in equipment technology have been achieved, so that DC is now on its way becoming the quasi-standard for the simple, fast, and sterile preparation of lipid-nanoparticles in small and medium batch sizes, including the possibility of simple and fast formulation screening or doing bed-side preparations of therapeutic nanoparticles.

Preparing liposomes is the most prominent application of DC so far, which can be explained by the exceptional simplicity and speed of the process. Other contributing factors are the scalability of the production process, the high level of safety provided by completely closed vessels, and the consistently very high entrapment efficiencies for virtually all substances.

This seminar will give an overview of the basic DC-processes and its use for controlled preparing of different types of liposomes, including SUV as well as small multilamellar vesicles (SMV).

Here you can  download the presentation.